Copper ion / H₂O₂ oxidation of Cu/Zn-Superoxide dismutase: Implications for enzymatic activity and antioxidant action.

ABSTRACT: Copper ion-catalyzed oxidation of yeast SOD1 (ySOD1) was examined to determine early oxidative modifications, including oxidation of a crucial disulfide bond, and the structural and functional repercussions of these events. The study used distinct oxidative conditions: Cu²⁺/H₂O₂, Cu²⁺/H₂O₂/AscH^- and Cu²⁺/H₂O₂/glucose. Capillary electrophoresis experiments and quantification of protein carbonyls indicate that ySOD1 is highly susceptible to oxidative modification and that changes can be detected within 0.1 min of the initiation of the reaction. Oxidation-induced structural perturbations, characterized by circular dichroism, revealed the formation of partially-unfolded ySOD1 species i

SUBMITTER: Tiwari MK 

PROVIDER: S-EPMC6614508 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature