Yeast applied readthrough inducing system (YARIS): an invivo assay for the comprehensive study of translational readthrough.

ABSTRACT: Stop codon readthrough-the decoding of a stop codon by a near-cognate tRNA-is employed by viruses to balance levels of enzymatic and structural proteins and by eukaryotic cells to enable isoform-specific protein synthesis in response to external stimuli. Owing to the prevalence of premature termination codons in human disease, readthrough has emerged as an attractive therapeutic target. A growing list of various features, for example the +4 nucleotide immediately following the stop codon, modulate readthrough levels, underscoring the need for systematic investigation of readthrough. Here, we identified and described a complete group of yeast tRNAs that induce readthrough in the stop-codon tetranucleotide manner when overexpressed, designated readthrough-inducing tRNAs (rti-tRNAs). These rt

SUBMITTER: Beznoskova P 

PROVIDER: S-EPMC6614816 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature