Probing G-quadruplex topologies and recognition concurrently in real time and 3D using a dual-app nucleoside probe.

ABSTRACT: Comprehensive understanding of structure and recognition properties of regulatory nucleic acid elements in real time and atomic level is highly important to devise efficient therapeutic strategies. Here, we report the establishment of an innovative biophysical platform using a dual-app nucleoside analog, which serves as a common probe to detect and correlate different GQ structures and ligand binding under equilibrium conditions and in 3D by fluorescence and X-ray crystallography techniques. The probe (SedU) is composed of a microenvironment-sensitive fluorophore and an excellent anomalous X-ray scatterer (Se), which is assembled by attaching a selenophene ring at 5-position of 2'-deoxyuridine. SedU incorporated into the loop region of human telomeric DNA repeat fluorescently distinguished

SUBMITTER: Nuthanakanti A 

PROVIDER: S-EPMC6614846 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature