The Paf1 Complex Broadly Impacts the Transcriptome of Saccharomyces cerevisiae.

ABSTRACT: The Polymerase Associated Factor 1 complex (Paf1C) is a multifunctional regulator of eukaryotic gene expression important for the coordination of transcription with chromatin modification and post-transcriptional processes. In this study, we investigated the extent to which the functions of Paf1C combine to regulate the Saccharomyces cerevisiae transcriptome. While previous studies focused on the roles of Paf1C in controlling mRNA levels, here, we took advantage of a genetic background that enriches for unstable transcripts, and demonstrate that deletion of PAF1 affects all classes of Pol II transcripts including multiple classes of noncoding RNAs (ncRNAs). By conducting a de novo differential expression analysis independent of gene annotations, we found that Paf1 posi

SUBMITTER: Ellison MA 

PROVIDER: S-EPMC6614894 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature