Functional and mechanistic studies reveal MAGEA3 as a pro-survival factor in pancreatic cancer cells.

ABSTRACT:

Background

In the era of personalized therapy, functional annotation of less frequent genetic aberrations will be instrumental in adapting effective therapeutic in clinic. Overexpression of Melanoma associated antigen A3 (MAGEA3) is reported in certain pancreatic cancer (PCA) patients. The major objective of the current study was to investigate the functional role of MAGEA3 in pancreatic cancer cells (PCCs) growth and survival.

Methods

Using overexpression (tet-on regulated system and constitutive expression system) and knockdown (by siRNA and shRNA) approach, we dissected the mechanistic role of MAGEA3 in pancreatic cancer pathogenesis. We generated MAGEA3 expressing stable PCA cell lines and mouse primary pancreatic epithelial cells. MAGEA3 was also depleted in certain MAG

SUBMITTER: Das B 

PROVIDER: S-EPMC6615156 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature