Deletion of Tet proteins results in quantitative disparities during ESC differentiation partially attributable to alterations in gene expression.

ABSTRACT:

Background

The Tet protein family (Tet1, Tet2, and Tet3) regulate DNA methylation through conversion of 5-methylcytosine to 5-hydroxymethylcytosine which can ultimately result in DNA demethylation and play a critical role during early mammalian development and pluripotency. While multiple groups have generated knockouts combining loss of different Tet proteins in murine embryonic stem cells (ESCs), differences in genetic background and approaches has made it difficult to directly compare results and discern the direct mechanism by which Tet proteins regulate the transcriptome. To address this concern, we utilized genomic editing in an isogenic pluripotent background which permitted a quantitative, flow-cytometry based measurement of pluripotency in combination with genome-wide asse

SUBMITTER: Reimer M 

PROVIDER: S-EPMC6615237 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature