High-content screen in human pluripotent cells identifies miRNA-regulated pathways controlling pluripotency and differentiation.

ABSTRACT:

Background

By post-transcriptionally regulating multiple target transcripts, microRNAs (miRNAs or miR) play important biological functions. H1 embryonic stem cells (hESCs) and NTera-2 embryonal carcinoma cells (ECCs) are two of the most widely used human pluripotent model cell lines, sharing several characteristics, including the expression of miRNAs associated to the pluripotent state or with differentiation. However, how each of these miRNAs functionally impacts the biological properties of these cells has not been systematically evaluated.

Methods

We investigated the effects of 31 miRNAs on NTera-2 and H1 hESCs, by transfecting miRNA mimics. Following 3-4 days of culture, cells were stained for the pluripotency marker OCT4 and the G2 cell-cycle marker Cyclin B1, and nucle

SUBMITTER: de Souza Lima IM 

PROVIDER: S-EPMC6615276 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature