Inferring the distribution of fitness effects of spontaneous mutations in Chlamydomonas reinhardtii.

ABSTRACT: Spontaneous mutations are the source of new genetic variation and are thus central to the evolutionary process. In molecular evolution and quantitative genetics, the nature of genetic variation depends critically on the distribution of effects of mutations on fitness and other quantitative traits. Spontaneous mutation accumulation (MA) experiments have been the principal approach for investigating the overall rate of occurrence and cumulative effect of mutations but have not allowed the phenotypic effects of individual mutations to be studied directly. Here, we crossed MA lines of the green alga Chlamydomonas reinhardtii with its unmutated ancestral strain to create haploid recombinant lines, each carrying an average of 50% of the accumulated mutations in a large number of combinations. Wi

SUBMITTER: Bondel KB 

PROVIDER: S-EPMC6615632 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature