Performance of recombinant chimeric proteins in the serological diagnosis of Trypanosoma cruzi infection in dogs.

ABSTRACT:

Background

Dogs are considered sentinels in areas of Trypanosoma cruzi transmission risk to humans. ELISA is generally the method of choice for diagnosing T. cruzi exposure in dogs, but its performance substantially depends on the antigenic matrix employed. In previous studies, our group has developed four chimeric antigens (IBMP-8.1, 8.2, 8.3, and 8.4) and evaluated their potential for diagnosing T. cruzi exposure in humans. For human sera, these chimeric antigens presented superior diagnostic performances as compared to commercial tests available in Brazil, Spain, and Argentina. Therefore, in this study we have evaluated the potential of these antigenic proteins for detection of anti-T. cruzi IgG antibodies in dog sera.

Methodology/principal findings

The IBMP-ELISA assays

SUBMITTER: Leony LM 

PROVIDER: S-EPMC6615644 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature