Control of p53-dependent transcription and enhancer activity by the p53 family member p63.

ABSTRACT: Transcriptional activation by p53 provides powerful, organism-wide tumor suppression. We hypothesized that the local chromatin environment, including differential enhancer activities, contributes to various p53-dependent transcriptional activities in different cell types during stress-induced signaling. In this work, using ChIP-sequencing, immunoblotting, quantitative PCR, and computational analyses across various mammalian cell lines, we demonstrate that the p53-induced transcriptome varies by cell type, reflects cell type-specific activities, and is considerably broader than previously anticipated. We found that these molecular events are strongly influenced by p53's engagement with differentially active cell type-specific enhancers and promoters. We also observed that p53 activity depen

SUBMITTER: Karsli Uzunbas G 

PROVIDER: S-EPMC6615668 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature