Characterization of two engineered dimeric Zika virus envelope proteins as immunogens for neutralizing antibody selection and vaccine design.

ABSTRACT: The envelope protein of Zika virus (ZIKV) exists as a dimer on the mature viral surface and is an attractive antiviral target because it mediates viral entry. However, recombinant soluble wild-type ZIKV envelope (wtZE) might preferentially exist as monomer (monZE). Recently, it has been shown that the A264C substitution could promote formation of dimeric ZIKV envelope protein (ZE_A264C), requiring further characterization of purified ZE_A264C for its potential applications in vaccine development. We also noted that ZE_A264C, connected by disulfide bond, might be different from the noncovalent native envelope dimer on the virion surface. Because the antibody Fc fragment exists as dimer and is widely used for fusion protein construction, here we fused wtZE to hu

SUBMITTER: Yang C 

PROVIDER: S-EPMC6615672 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature