Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.

ABSTRACT: In vitro models of the small intestine are crucial tools for the prediction of drug absorption. The Caco-2 monolayer transwell model has been widely employed to assess drug absorption across the intestine. However, it is now well-established that 3D in vitro models capture tissue-specific architecture and interactions with the extracellular matrix and therefore better recapitulate the complex in vivo environment. However, these models need to be characterized for barrier properties and changes in gene expression and transporter function. Here, we report that geometrically controlled self-assembling multicellular intestinal Caco-2 spheroids cultured using Sacrificial Micromolding display reproducible intestinal features and functions that are more representative of the in vivo small intesti

SUBMITTER: Samy KE 

PROVIDER: S-EPMC6616551 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature