Interrogation of Phenotypic Plasticity between Epithelial and Mesenchymal States in Breast Cancer.

ABSTRACT: Dynamic interconversions between transitional epithelial and mesenchymal states underpin the epithelial mesenchymal plasticity (EMP) seen in some carcinoma cell systems. We have delineated epithelial and mesenchymal subpopulations existing within the PMC42-LA breast cancer cell line by their EpCAM expression. These purified but phenotypically plastic states, EpCAM^High (epithelial) and EpCAM^Low (mesenchymal), have the ability to regain the phenotypic equilibrium of the parental population (i.e., 80% epithelial and 20% mesenchymal) over time, although the rate of reversion in the mesenchymal direction (epithelial-mesenchymal transition; EMT) is higher than that in the epithelial direction (mesenchymal-epithelial transition; MET). Single-cell clonal propagation was imple

SUBMITTER: Bhatia S 

PROVIDER: S-EPMC6617164 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature