Investigation of candidate genes of non-syndromic cleft lip with or without cleft palate, using both case-control and family-based association studies.

ABSTRACT:

Objective

Non-syndromic cleft of the lip and/or palate (NSCL/P) is one of the most common polygenic diseases. In this study, both case-control and family-based association study were used to confirm whether the Single Nucleotide Polymorphisms (SNPs) were associated with NSCL/P.

Methods

A total of 37 nuclear families and 189 controls were recruited, whose blood DNA was extracted and subjected to genotyping of SNPs of 27 candidate genes by polymerase chain reaction-improved multiple ligase detection reaction technology (PCR-iMLDR). Case-control statistical analysis was performed using the SPSS 19.0. Haplotype Relative Risk (HRR), transmission disequilibrium test (TDT), and Family-Based Association Test (FBAT) were used to test for over-transmission of the target alleles in cas

SUBMITTER: Ge X 

PROVIDER: S-EPMC6617431 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature