Identification of prognosis markers for endometrial cancer by integrated analysis of DNA methylation and RNA-Seq data.

ABSTRACT: Endometrial cancer is highly malignant and has a poor prognosis in the advanced stage, thus, prediction of its prognosis is important. DNA methylation has rapidly gained clinical attention as a biomarker for diagnostic, prognostic and predictive purposes in various cancers. In present study, differentially methylated positions and differentially expressed genes were identified according to DNA methylation and RNA-Seq data. Functional analyses and interaction network were performed to identify hub genes, and overall survival analysis of hub genes were validated. The top genes were evaluated by immunohistochemical staining of endometrial cancer tissues. The gene function was evaluated by cell growth curve after knockdown CDC20 and CCNA2 of endometrial cancer cell line. A total of 329 hypomet

SUBMITTER: Huo X 

PROVIDER: S-EPMC6617448 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature