Quantitative proteomic analysis of sperm in unexplained recurrent pregnancy loss.

ABSTRACT:

Background

Recurrent pregnancy loss (RPL) refers to two or more spontaneous abortions that occur consecutively with the same spouse. RPL severely affects human reproduction health, and causes extreme physical and mental suffering to patients and their families.

Methods

We used isobaric tags for relative and absolute quantitation (iTRAQ), which was coupled with liquid chromatography mass spectrometry (LC-MS) proteomic analysis, in order to identify differentially expressed proteins. Moreover, we used western blot to analyze differentially expressed proteins.

Results

Of the 2350 non-redundant proteins identified, 38 proteins were significantly altered and were identified as potential biomarkers for RPL. The protein-protein interaction network constructed using GeneMANIA

SUBMITTER: Xue D 

PROVIDER: S-EPMC6617596 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature