Dataset Information

Interactive effects of nitrogen and potassium on photosynthesis and photosynthetic nitrogen allocation of rice leaves.

ABSTRACT:

Background

Nitrogen (N) and potassium (K) are two important mineral nutrients in regulating leaf photosynthesis. Studying the interactive effects of N and K on regulating N allocation and photosynthesis (P_n) of rice leaves will be of great significance for further increasing leaf P_n, photosynthetic N use efficiency (PNUE) and grain yield. We measured the gas exchange of rice leaves in a field experiment and tested different kinds of leaf N based on N morphology and function, and calculated the interactive effects of N and K on N allocation and the PNUE.

Results

Compared with N0 (0 kg N ha^- 1) and K0 (0 kg K₂O ha^- 1) treatments, the P_n was increased by 17.1 and 12.2% with the supply of N and K. Compared with N0K0 (0 kg N and 0 kg K₂O ha^- 1), N0K120 (0 kg N and 120 kg K₂O ha^- 1) and N0K180 (0 kg N and 180 kg K₂O ha^- 1), N supply increased the absolute content of photosynthetic N (N_psn) by 15.1, 15.5 and 10.5% on average, and the storage N (N_store) was increased by 32.7, 64.9 and 72.7% on average. The relative content of N_psn was decreased by 5.6, 12.1 and 14.5%, while that of N_store was increased by 8.7, 27.8 and 33.8%. Supply of K promoted the transformation of N_store to N_psn despite the leaf N content (N_a) was indeed decreased. Compared with N0K0, N180K0 (180 kg N and 0 kg K₂O ha^- 1) and N270K0 (270 kg N and 0 kg K₂O ha^- 1), K supply increased the relative content of N_psn by 17.7, 8.8 and 7.3%, and decreased the relative content of N_store by 24.2, 11.4 and 8.7% respectively.

Conclusions

This study indicated the mechanism that K supply decreased the N_a but increased the N_psn content and then increased leaf P_n and PNUE from a new viewpoint of leaf N allocation. The supply of K promoted the transformation of N_store to N_psn and increased the PNUE. The decreased N_store mainly resulted from the decrease of non-protein N. Combined use of N and K could optimize leaf N allocation and maintain a high leaf N_psn content and PNUE.

SUBMITTER: Hou W

PROVIDER: S-EPMC6617825 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

Interactive effects of nitrogen and potassium on photosynthesis and photosynthetic nitrogen allocation of rice leaves.

Hou Wenfeng W Tränkner Merle M Lu Jianwei J Yan Jinyao J Huang Siyuan S Ren Tao T Cong Rihuan R Li Xiaokun X

BMC plant biology 20190710 1

<h4>Background</h4>Nitrogen (N) and potassium (K) are two important mineral nutrients in regulating leaf photosynthesis. Studying the interactive effects of N and K on regulating N allocation and photosynthesis (P<sub>n</sub>) of rice leaves will be of great significance for further increasing leaf P<sub>n</sub>, photosynthetic N use efficiency (PNUE) and grain yield. We measured the gas exchange of rice leaves in a field experiment and tested different kinds of leaf N based on N morphology and ...[more]

PMID: 31291890

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Project description:BackgroundOver the past decades, the structural and functional genomics of rice have been deeply studied, and high density of molecular genetic markers have been developed. However, the genetic variation in leaf photosynthesis, the most important trait for rice yield improvement, was rarely studied. The lack of photosynthesis phenotyping tools is one of the bottlenecks, as traditional direct photosynthesis measurements are very low-throughput, and recently developed high-throughput methods are indirect measurements. Hence, there is an urgent need for a fast, accurate and direct measurement approach.ResultWe reported a fast photosynthesis measurement (FPM) method for phenotyping photosynthetic capacity of rice, which measures photosynthesis of excised tillers in environment-controlled lab conditions. The light response curves measured using FPM approach coped well with that the curves measured using traditional gas exchange approach. Importantly, the FPM technique achieved an average throughput of 5.4 light response curves per hour, which was 3 times faster than the 1.8 light response curves per hour using the traditional method. Tillers sampled at early morning had the highest photosynthesis, stomatal conductance and the lowest variability. In addition, even 12 h after sampling, there was no significant difference of photosynthesis rate between excised tillers and in situ. We finally investigated the genetic variations of photosynthetic traits across 568 F2 lines using the FPM technique and discussed the logistics of screening several hundred samples per day per instrumental unit using FPM to generate a wealth of photosynthetic phenotypic data, which might help to improve the selection power in large populations of rice with the ultimate aim of improving yield through improved photosynthesis.ConclusionsHere we developed a high-throughput method that can measure the rice leaf photosynthetic capacity approximately 10 times faster than traditional gas exchange approaches. Importantly, this method can overcome measurement errors caused by environmental heterogeneity under field conditions, and it is possible to measure 12 or more hours per day under lab conditions.

Project description:It has been theorized that photosynthetic radiation use efficiency (PhRUE) over the course of a day is constant for leaves throughout a canopy if leaf nitrogen content and photosynthetic properties are adapted to local light so that canopy photosynthesis over a day is optimized. To test this hypothesis, 'daily' photosynthesis of individual leaves of Solanum melongena plants was calculated from instantaneous rates of photosynthesis integrated over the daylight hours. Instantaneous photosynthesis was estimated from the photosynthetic responses to photosynthetically active radiation (PAR) and from the incident PAR measured on individual leaves during clear and overcast days. Plants were grown with either abundant or scarce N fertilization. Both net and gross daily photosynthesis of leaves were linearly related to daily incident PAR exposure of individual leaves, which implies constant PhRUE over a day throughout the canopy. The slope of these relationships (i.e. PhRUE) increased with N fertilization. When the relationship was calculated for hourly instead of daily periods, the regressions were curvilinear, implying that PhRUE changed with time of the day and incident radiation. Thus, linearity (i.e. constant PhRUE) was achieved only when data were integrated over the entire day. Using average PAR in place of instantaneous incident PAR increased the slope of the relationship between daily photosynthesis and incident PAR of individual leaves, and the regression became curvilinear. The slope of the relationship between daily gross photosynthesis and incident PAR of individual leaves increased for an overcast compared with a clear day, but the slope remained constant for net photosynthesis. This suggests that net PhRUE of all leaves (and thus of the whole canopy) may be constant when integrated over a day, not only when the incident PAR changes with depth in the canopy, but also when it varies on the same leaf owing to changes in daily incident PAR above the canopy. The slope of the relationship between daily net photosynthesis and incident PAR was also estimated from the photosynthetic light response curve of a leaf at the top of the canopy and from the incident PAR above the canopy, in place of that measured on individual leaves. The slope (i.e. net PhRUE) calculated in this simple way did not differ statistically from that calculated using data from individual leaves.

Dataset Information

Interactive effects of nitrogen and potassium on photosynthesis and photosynthetic nitrogen allocation of rice leaves.

Background

Results

Conclusions

Publications

Interactive effects of nitrogen and potassium on photosynthesis and photosynthetic nitrogen allocation of rice leaves.

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