Dataset Information

Comparative transcriptome analysis of two reproductive modes in Adiantum reniforme var. sinense targeted to explore possible mechanism of apogamy.

ABSTRACT:

Background

Apogamy is a unique asexual reproduction in the ferns, in which somatic cells of gametophytes go through dedifferentiation and then differentiate into haploid sporophytes bypassing fertilization. Restricted to the lack of genomic information, molecular mechanisms of apogamy have remained unclear. Comparative transcriptome analysis was conducted at six stages between sexual reproduction and apogamy in the fern Adiantum reniforme var. sinense, in an effort to identify genes and pathways that might initiate the asexual reproduction.

Results

Approximately 928 million high-quality clean reads were assembled into 264,791 unigenes with an average length of 615 bp. A total of 147,865 (55.84%) unigenes were successfully annotated. Differential genes expression analysis indicated that transcriptional regulation was more active in the early stage of apogamy compared to sexual reproduction. Further comparative analysis of the enriched pathways between the early stages of the two reproductive modes demonstrated that starch and sucrose metabolism pathway responsible for cell wall was only significantly enriched in asexual embryonic cell initiation. Furthermore, regulation of plant hormone related genes was more vigorous in apogamy initiation.

Conclusion

These findings would be useful for revealing the initiation of apogamy and further understanding of the mechanisms related to asexual reproduction.

SUBMITTER: Fu Q

PROVIDER: S-EPMC6617869 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Publications

Comparative transcriptome analysis of two reproductive modes in Adiantum reniforme var. sinense targeted to explore possible mechanism of apogamy.

Fu Qi Q Chen Long-Qing LQ

BMC genetics 20190709 1

<h4>Background</h4>Apogamy is a unique asexual reproduction in the ferns, in which somatic cells of gametophytes go through dedifferentiation and then differentiate into haploid sporophytes bypassing fertilization. Restricted to the lack of genomic information, molecular mechanisms of apogamy have remained unclear. Comparative transcriptome analysis was conducted at six stages between sexual reproduction and apogamy in the fern Adiantum reniforme var. sinense, in an effort to identify genes and ...[more]

PMID: 31288742

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Project description:BackgroundThere are different opinions about the relationship of two disjunctively distributed varieties Adiantum reniforme L. var. sinense Y.X.Lin and Adiantum reniforme L. Adiantum reniforme var. sinense is an endangered fern only distributed in a narrowed region of Chongqing city in China, while Adiantum reniforme var. reniforme just distributed in Canary Islands and Madeira off the north-western African coast. To verify the relationship of these two taxa, relative phylogenetic analyses, karyotype analyses, microscopic spore observations and morphological studies were performed in this study. Besides, divergence time between A. reniforme var. sinense and A. reniforme var. reniforme was estimated using GTR model according to a phylogeny tree constructed with the three cpDNA markers atpA, atpB, and rbcL.ResultsPhylogenetic results and divergence time analyses--all individuals of A. reniforme var. sinense from 4 different populations (representing all biogeographic distributions) were clustered into one clade and all individuals of A. reniforme var. reniforme from 7 different populations (all biogeographic distributions are included) were clustered into another clade. The divergence between A. reniforme var. reniforme and A. reniforme var. sinense was estimated to be 4.94 (2.26-8.66) Myr. Based on karyotype analyses, A. reniforme var. reniforme was deduced to be hexaploidy with 2n = 180, X = 30, while A. reniforme var. sinense was known as tetraploidy. Microscopic spore observations suggested that surface ornamentation of A. reniforme var. reniforme is psilate, but that of A. reniforme var. sinense is rugate. Leaf blades of A. reniforme var. sinense are membranous and reniform and with several obvious concentric rings, and leaves of A. reniforme var. reniforme are pachyphyllous and coriaceous and are much rounder and similar to palm.ConclusionAdiantum reniforme var. sinense is an independent species rather than the variety of Adiantum reniforme var. reniforme. As a result, we approve Adiantum nelumboides X. C. Zhang, nom. & stat. nov. as a legal name instead of the former Adiantum reniforme var. sinense. China was determined to be the most probable evolution centre based on the results of phylogenetic analyses, divergence estimation, relative palaeogeography and palaeoclimate materials.

Dataset Information

Comparative transcriptome analysis of two reproductive modes in Adiantum reniforme var. sinense targeted to explore possible mechanism of apogamy.

Background

Results

Conclusion

Publications

Comparative transcriptome analysis of two reproductive modes in Adiantum reniforme var. sinense targeted to explore possible mechanism of apogamy.

Similar Datasets

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