Newly designed 16S rRNA metabarcoding primers amplify diverse and novel archaeal taxa from the environment.

ABSTRACT: High-throughput studies of microbial communities suggest that Archaea are a widespread component of microbial diversity in various ecosystems. However, proper quantification of archaeal diversity and community ecology remains limited, as sequence coverage of Archaea is usually low owing to the inability of available prokaryotic primers to efficiently amplify archaeal compared to bacterial rRNA genes. To improve identification and quantification of Archaea, we designed and validated the utility of several primer pairs to efficiently amplify archaeal 16S rRNA genes based on up-to-date reference genes. We demonstrate that several of these primer pairs amplify phylogenetically diverse Archaea with high sequencing coverage, outperforming commonly used primers. Based on comparing the resulting l

SUBMITTER: Bahram M 

PROVIDER: S-EPMC6618113 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature