MyROOT: a method and software for the semiautomatic measurement of primary root length in Arabidopsis seedlings.

ABSTRACT: Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we developed MyROOT, a software for the semiautomatic quantification of root growth of seedlings growing directly on agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom-up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabid

SUBMITTER: Betegon-Putze I 

PROVIDER: S-EPMC6618301 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature