Deep supercooling enabled by surface impregnation with lipophilic substances explains the survival of overwintering buds at extreme freezing.

ABSTRACT: The frost survival mechanism of vegetative buds of angiosperms was suggested to be extracellular freezing causing dehydration, elevated osmotic potential to prevent freezing. However, extreme dehydration would be needed to avoid freezing at the temperatures down to -45°C encountered by many trees. Buds of Alnus alnobetula, in common with other frost hardy angiosperms, excrete a lipophilic substance, whose functional role remains unclear. Freezing of buds was studied by infrared thermography, psychrometry, and cryomicroscopy. Buds of A. alnobetula did not survive by extracellular ice tolerance but by deep supercooling, down to -45°C. An internal ice barrier prevented ice penetration from the frozen stem into the bud. Cryomicroscopy revealed a new freezing mechanism. Until now, supercooled b

SUBMITTER: Neuner G 

PROVIDER: S-EPMC6618773 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature