Elastomeric sensor surfaces for high-throughput single-cell force cytometry.

ABSTRACT: As cells with aberrant force-generating phenotypes can directly lead to disease, cellular force-generation mechanisms are high-value targets for new therapies. Here, we show that single-cell force sensors embedded in elastomers enable single-cell force measurements with ~100-fold improvement in throughput than was previously possible. The microtechnology is scalable and seamlessly integrates with the multi-well plate format, enabling highly parallelized time-course studies. In this regard, we show that airway smooth muscle cells isolated from fatally asthmatic patients have innately greater and faster force-generation capacity in response to stimulation than healthy control cells. By simultaneously tracing agonist-induced calcium flux and contractility in the same cell, we show that the ca

SUBMITTER: Pushkarsky I 

PROVIDER: S-EPMC6619436 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature