Leaf-induced callus formation in two cultivars: hot pepper 'CM334' and bell pepper 'Dempsey'.

ABSTRACT: Pepper (Capsicum annuum), one of the most economically important vegetables of the Solanaceae family, is cultivated worldwide. To apply versatile genome-editing tools to a pepper genome for precise molecular breeding, an in vitro regeneration protocol is indispensable and callus formation is an essential step in the regeneration of pepper. Here, we show that calli were successfully induced from young leaves (3-4 cm) of pepper plants, the hot pepper C. annum 'CM334' ('CM334') and bell pepper C. annum 'Dempsey' ('Dempsey'), grown on soil for less than 7 weeks. The excised leaf segments of 'CM334' produced white calli in B5 medium containing 3% sucrose (3S), 2 mg/L 6-benzylaminopurine (2BAP), and 1 mg/L α-naphthalene acetic acid (1NAA). The calli were able t

SUBMITTER: Kim H 

PROVIDER: S-EPMC6619979 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature