An efficient Agrobacterium-mediated soybean transformation method using green fluorescent protein as a selectable marker.

ABSTRACT: Genetic transformation plays a vital role in gene functional study and molecular breeding of soybean. Conventional soybean transformation methods using chemical selectable markers, such as antibiotic or herbicide resistance genes, rely on the identification of positive transgenic lines at advanced developmental stages, making selection procedure labor intensive and time consuming. Utilization of a visual maker to track the transgene would avoid the uncertainty and blindness in the transformation process. In this research, we used green fluorescent protein (GFP) as the selectable marker to detect transgenics at early stages of soybean development. Positive transformants were detected recurrently during each stage of the process based on visualization of the green fluorescence signal, which

SUBMITTER: Yang S 

PROVIDER: S-EPMC6619996 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature