Conformational change within the extracellular domain of B cell receptor in B cell activation upon antigen binding.

ABSTRACT: B lymphocytes use B cell receptors (BCRs) to recognize antigens. It is still not clear how BCR transduces antigen-specific physical signals upon binding across cell membrane for the conversion to chemical signals, triggering downstream signaling cascades. It is hypothesized that through a series of conformational changes within BCR, antigen engagement in the extracellular domain of BCR is transduced to its intracellular domain. By combining site-specific labeling methodology and FRET-based assay, we monitored conformational changes in the extracellular domains within BCR upon antigen engagement. Conformational changes within heavy chain of membrane-bound immunoglobulin (mIg), as well as conformational changes in the spatial relationship between mIg and Igβ were observed. These conformation

SUBMITTER: Shen Z 

PROVIDER: S-EPMC6620044 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature