The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings.

ABSTRACT: Previously, through a TILLING (Targeting Induced Local Lesions in Genomes) approach applied on barley chloroplast mutator (cpm) seedlings a high frequency of polymorphisms in the rpl23 gene was detected. All the polymorphisms corresponded to five differences already known to exist in nature between the rpl23 gene located in the inverted repeats (IRs) and the rpl23 pseudogene located in the large single copy region (LSC). In this investigation, polymorphisms in the rpl23 gene were verified and besides, a similar situation was found for the pseudogene in cpm seedlings. On the other hand, no polymorphisms were found in any of those loci in 40 wild type barley seedlings. Those facts and the independent occurrence of polymorphisms in the gene and pseudogene in individual seedlings suggest that

SUBMITTER: Lencina F 

PROVIDER: S-EPMC6620283 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature