Translational and transcriptional responses in human primary hepatocytes under hypoxia.

ABSTRACT: The liver is the primary source of a large number of plasma proteins and plays a critical role in multiple biological processes. Inadequate oxygen supply characterizing various clinical settings such as liver transplantation exposes the liver to hypoxic conditions. Studies assessing hypoxia-induced global translational changes in liver are lacking. Here, we employed a recently developed ribosome-profiling technique to assess global translational responses of human primary hepatocytes exposed to acute hypoxic stress (1% O2) for the short term. In parallel, transcriptome profiling was performed to assess mRNA expression changes. We found that translational responses appeared earlier and were predominant over transcriptional responses. A significant decrease in translational efficiency of sev

SUBMITTER: Hettiarachchi GK 

PROVIDER: S-EPMC6620582 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature