Characterization of Antigenic Oligosaccharides from Gram-Negative Bacteria via Activated Electron Photodetachment Mass Spectrometry.

ABSTRACT: Lipooligosaccharides (LOS), composed of hydrophilic oligosaccharides and hydrophobic lipid A domains, are found on the outer membranes of Gram-negative bacteria. Here we report the characterization of deacylated LOS of LPS by activated-electron photodetachment mass spectrometry. Collision induced dissociation (CID) of these phosphorylated oligosaccharides produces simple MS/MS spectra with most fragment ions arising from cleavages near the reducing end of the molecule where the phosphate groups are located. In contrast, 193 nm ultraviolet photodissociation (UVPD) generates a wide array of product ions throughout the oligosaccharide including cross-ring fragments that illuminate the branching patterns. However, there are also product ions that are redundant or uninformative, resulting in mo

SUBMITTER: Crittenden CM 

PROVIDER: S-EPMC6620780 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature