Exploration of CRISPR/Cas9-based gene editing as therapy for osteoarthritis.

ABSTRACT:

Objectives

Osteoarthritis (OA) is a painful and debilitating disease and it is associated with aberrant upregulation of multiple factors, including matrix metalloproteinase 13 (MMP13), interleukin-1β (IL-1β) and nerve growth factor (NGF). In this study, we aimed to use the CRISPR/Cas9 technology, a highly efficient gene-editing tool, to study whether the ablation of OA-associated genes has OA-modifying effects.

Methods

We performed intra-articular injection of adeno-associated virus, which expressed CRISPR/Cas9 components to target each of the genes encoding MMP13, IL-1β and NGF, in a surgically induced OA mouse model. We also tested triple ablations of NGF, MMP13 and IL-1β.

Results

Loss-of-function of NGF palliates pain but worsens joint damage in the surgically indu

SUBMITTER: Zhao L 

PROVIDER: S-EPMC6621547 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature