Meclofenamic acid represses spermatogonial proliferation through modulating m⁶A RNA modification.

ABSTRACT:

Background

N6-Methyladenosine (m⁶A), the most prevalent modification in mammalian mRNA, plays important roles in numerous biological processes. Several m⁶A associated proteins such as methyltransferase like 3 (METTL3), methyltransferase like 14 (METTL14), α-ketoglutarate-dependent dioxygenase AlkB homolog 5 (ALKBH5) and YTH domain containing 2 (YTHDC2) are involved in the regulation of spermatogenesis and oogenesis. However, the role of the first detected m6A demethylase, fat mass and obesity associate protein (FTO), in germ cells remains elusive. Elucidation of FTO roles in the regulation of germ cell fate will provide novel insights into the mammalian reproduction.

Methods

Mouse GC-1 spg cells were treated with the ester form of meclofenamic acid (MA2

SUBMITTER: Huang T 

PROVIDER: S-EPMC6621992 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature