Pan-Genomic and Polymorphic Driven Prediction of Antibiotic Resistance in Elizabethkingia.

ABSTRACT: The Elizabethkingia are a genetically diverse genus of emerging pathogens that exhibit multidrug resistance to a range of common antibiotics. Two representative species, Elizabethkingia bruuniana and E. meningoseptica, were phenotypically tested to determine minimum inhibitory concentrations (MICs) for five antibiotics. Ultra-long read sequencing with Oxford Nanopore Technologies (ONT) and subsequent de novo assembly produced complete, gapless circular genomes for each strain. Alignment based annotation with Prokka identified 5,480 features in E. bruuniana and 5,203 features in E. meningoseptica, where none of these identified genes or gene combinations corresponded to observed phenotypic resistance values. Pan-genomic analysis, performed with an a

SUBMITTER: Naidenov B 

PROVIDER: S-EPMC6622151 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature