Evolutionary couplings detect side-chain interactions.

ABSTRACT: Patterns of amino acid covariation in large protein sequence alignments can inform the prediction of de novo protein structures, binding interfaces, and mutational effects. While algorithms that detect these so-called evolutionary couplings between residues have proven useful for practical applications, less is known about how and why these methods perform so well, and what insights into biological processes can be gained from their application. Evolutionary coupling algorithms are commonly benchmarked by comparison to true structural contacts derived from solved protein structures. However, the methods used to determine true structural contacts are not standardized and different definitions of structural contacts may have important consequences for interpreting the results from evolutiona

SUBMITTER: Hockenberry AJ 

PROVIDER: S-EPMC6622159 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature