Tyrosine Phosphorylation of CD2AP Affects Stability of the Slit Diaphragm Complex.

ABSTRACT:

Background

CD2-associated protein (CD2AP), a slit diaphragm-associated scaffolding protein involved in survival and regulation of the cytoskeleton in podocytes, is considered a "stabilizer" of the slit diaphragm complex that connects the slit diaphragm protein nephrin to the cytoskeleton of the cell. Tyrosine phosphorylation of slit diaphragm molecules can influence their surface expression, but it is unknown whether tyrosine phosphorylation events of CD2AP are also physiologically relevant to slit diaphragm stability.

Methods

We used isoelectric focusing, western blot analysis, and immunofluorescence to investigate phosphorylation of CD2AP, and phospho-CD2AP antibodies and site-directed mutagenesis to define the specific phosphorylated tyrosine residues. We used cross-speci

SUBMITTER: Tossidou I 

PROVIDER: S-EPMC6622410 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature