Dissection of the IgE and T-cell recognition of the major group 5 grass pollen allergen Phl p 5.

ABSTRACT:

Background

The major timothy grass pollen allergen Phl p 5 belongs to the most potent allergens involved in hay fever and asthma.

Objective

This study characterized immune-dominant IgE- and T-cell-recognition sites of Phl p 5.

Methods

Seven peptides, P1 to P7 with a length of 31 to 38 amino acids that spanned the Phl p 5 sequence, were synthesized, characterized by circular dichroism spectroscopy, and tested for IgE reactivity, basophil activation, and T-cell reactivity. Carrier-bound peptides were studied for their ability to induce IgG antibodies in rabbits which recognize Phl p 5 or cross-reactive allergens from different grass species. Peptide-specific antibodies were tested for the capability to inhibit IgE reactivity to Phl p 5 and allergen-induced basophil acti

SUBMITTER: Focke-Tejkl M 

PROVIDER: S-EPMC6624141 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature