Exploring Wells-Dawson Clusters Associated With the Small Ribosomal Subunit.

ABSTRACT: The polyoxometalate P₂W₁₈ O 62 6 - , the Wells-Dawson cluster, stabilized the ribosome sufficiently for the crystallographers to solve the phase problem and improve the structural resolution. In the following we characterize the interaction of the Wells-Dawson cluster with the ribosome small subunit. There are 14 different P₂W₁₈ O 62 6 - clusters interacting with the ribosome, and the types of interactions range from one simple residue interaction to complex association of multiple sites including backbone interactions with a Wells-Dawson cluster. Although well-documented that bridging oxygen atoms are the main basic sites on other polyoxometalate interaction with most proteins reported, the W=O groups are the main sites of the Wells-Dawson

SUBMITTER: Crans DC 

PROVIDER: S-EPMC6624422 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature