Interferon-microRNA signalling drives liver precancerous lesion formation and hepatocarcinogenesis.

ABSTRACT:

Objective

Precancerous lesion, a well-established histopathologically premalignant tissue with the highest risk for tumourigenesis, develops preferentially from activation of DNA damage checkpoint and persistent inflammation. However, little is known about the mechanisms by which precancerous lesions are initiated and their physiological significance.

Design

Laser capture microdissection was used to acquire matched normal liver, precancerous lesion and tumour tissues. miR-484(-/-), Ifnar1(-/-) and Tgfbr2(△hep) mice were employed to determine the critical role of the interferon (IFN)-microRNA pathway in precancerous lesion formation and tumourigenesis. RNA immunoprecipitation (RIP), pull-down and chromatin immunoprecipitation (ChIP) assays were applied to explore the underlyi

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6624432 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature