A novel model of controlling PD-L1 expression in ALK⁺ anaplastic large cell lymphoma revealed by CRISPR screening.

ABSTRACT: The success of programmed cell death protein 1 (PD-1)/PD-L1-based immunotherapy highlights the critical role played by PD-L1 in cancer progression and reveals an urgent need to develop new approaches to attenuate PD-L1 function by gaining insight into how its expression is controlled. Anaplastic lymphoma kinase (ALK)-positive anaplastic large-cell lymphoma (ALK⁺ ALCL) expresses a high level of PD-L1 as a result of the constitutive activation of multiple oncogenic signaling pathways downstream of ALK activity, making it an excellent model in which to define the signaling processes responsible for PD-L1 upregulation in tumor cells. Here, using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 library screening, we sought a comprehensive understanding of the m

SUBMITTER: Zhang JP 

PROVIDER: S-EPMC6624970 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature