High glucose microenvironment accelerates tumor growth via SREBP1-autophagy axis in pancreatic cancer.

ABSTRACT:

Background

Diabetes is recognized to be a risk factor of pancreatic cancer, but the mechanism has not been fully elucidated. Sterol regulatory element binding protein 1 (SREBP1) is an important transcription factor involved in both lipid metabolism and tumor progression. However, the relationship between high glucose microenvironment, SREBP1 and pancreatic cancer remains to be explored.

Methods

Clinical data and surgical specimens were collected. Pancreatic cancer cell lines BxPc-3 and MiaPaCa-2 were cultured in specified medium. Immunohistochemistry (IHC) and western blotting were performed to detect the expression of SREBP1. MTT and colony formation assays were applied to investigate cell proliferation. Immunofluorescence, mRFP-GFP adenoviral vector and transmission electr

SUBMITTER: Zhou C 

PROVIDER: S-EPMC6625066 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature