Systematic evaluation of RNA-Seq preparation protocol performance.

ABSTRACT:

Background

RNA-Seq is currently the most widely used tool to analyze whole-transcriptome profiles. There are numerous commercial kits available to facilitate preparing RNA-Seq libraries; however, it is still not clear how some of these kits perform in terms of: 1) ribosomal RNA removal; 2) read coverage or recovery of exonic vs. intronic sequences; 3) identification of differentially expressed genes (DEGs); and 4) detection of long non-coding RNA (lncRNA). In RNA-Seq analysis, understanding the strengths and limitations of commonly used RNA-Seq library preparation protocols is important, as this technology remains costly and time-consuming.

Results

In this study, we present a comprehensive evaluation of four RNA-Seq kits. We used three standard input protocols: Illumina TruS

SUBMITTER: Chao HP 

PROVIDER: S-EPMC6625085 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature