Identification of key genes and upstream regulators in ischemic stroke.

ABSTRACT:

Introduction

Ischemic stroke (IS) causes severe neurological impairments and physical disabilities and has a high economic burden. Our study aims to identify the key genes and upstream regulators in IS by integrated microarray analysis.

Methods

An integrated analysis of microarray studies of IS was performed to identify the differentially expressed genes (DEGs) in IS compared to normal control. Based on these DEGs, we performed the functional annotation and transcriptional regulatory network constructions. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the expression of DEGs.

Results

From two Gene Expression Omnibus datasets obtained, we obtained 1526 DEGs (534 up-regulated and 992 down-regulated genes) between IS and normal control

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6625467 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature