High-Affinity Bent β₂-Integrin Molecules in Arresting Neutrophils Face Each Other through Binding to ICAMs In cis.

ABSTRACT: Leukocyte adhesion requires β₂-integrin activation. Resting integrins exist in a bent-closed conformation-i.e., not extended (E^-) and not high affinity (H^-)-unable to bind ligand. Fully activated E⁺H⁺ integrin binds intercellular adhesion molecules (ICAMs) expressed on the opposing cell in trans. E^-H^- transitions to E⁺H⁺ through E⁺H^- or through E^-H⁺, which binds to ICAMs on the same cell in cis. Spatial patterning of activated integrins is thought to be required for effective arrest, but no high-resolution cell surface localization maps of activated integrins exist. Here, we developed Super-STORM by combining super-resolution microscopy with molecul

SUBMITTER: Fan Z 

PROVIDER: S-EPMC6625519 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature