Temporal Variability and Stability of the Fecal Microbiome: The Multiethnic Cohort Study.

ABSTRACT:

Background

Measurement reliability and biological stability need to be considered when developing sampling protocols for population-based fecal microbiome studies.

Methods

Stool samples were collected biannually over a 2-year period and sequenced for the V1-V3 region of the 16S rRNA gene in 50 participants from the Multiethnic Cohort Study. We evaluated the temporal stability of the fecal microbiome on a community level with permutational multivariate analysis of variance (PERMANOVA), as well as on taxa and diversity measures with intraclass correlation coefficients.

Results

Interindividual differences were the predominant source of fecal microbiome variation, and variation within individual was driven more by changing abundances than by the complete loss or introduct

SUBMITTER: Fu BC 

PROVIDER: S-EPMC6625800 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature