Arabidopsis FLL2 promotes liquid-liquid phase separation of polyadenylation complexes.

ABSTRACT: An important component of cellular biochemistry is the concentration of proteins and nucleic acids in non-membranous compartments^1,2. These biomolecular condensates are formed from processes that include liquid-liquid phase separation. The multivalent interactions necessary for liquid-liquid phase separation have been extensively studied in vitro^1,3. However, the regulation of this process in vivo is poorly understood. Here we identify an in vivo regulator of liquid-liquid phase separation through a genetic screen targeting factors required for Arabidopsis RNA-binding protein FCA function. FCA contains prion-like domains that phase-separate in vitro, and exhibits behaviour in vivo that is consistent with phase separation. The mutant screen identified a functional requ

SUBMITTER: Fang X 

PROVIDER: S-EPMC6625965 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature