Ontology highlight
ABSTRACT:
SUBMITTER: Lentini A
PROVIDER: S-EPMC6625966 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Nature methods 20180625 7
DNA immunoprecipitation followed by sequencing (DIP-seq) is a common enrichment method for profiling DNA modifications in mammalian genomes. However, the results of independent DIP-seq studies often show considerable variation between profiles of the same genome and between profiles obtained by alternative methods. Here we show that these differences are primarily due to the intrinsic affinity of IgG for short unmodified DNA repeats. This pervasive experimental error accounts for 50-99% of regio ...[more]