Generating kinetic environments to study dynamic cellular processes in single cells.

ABSTRACT: Cells of any organism are consistently exposed to changes over time in their environment. The kinetics by which these changes occur are critical for the cellular response and fate decision. It is therefore important to control the temporal changes of extracellular stimuli precisely to understand biological mechanisms in a quantitative manner. Most current cell culture and biochemical studies focus on instant changes in the environment and therefore neglect the importance of kinetic environments. To address these shortcomings, we developed two experimental methodologies to precisely control the environment of single cells. These methodologies are compatible with standard biochemistry, molecular, cell and quantitative biology assays. We demonstrate applicability by obtaining time series and

SUBMITTER: Thiemicke A 

PROVIDER: S-EPMC6625993 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature