Preparation and characterization of a single-domain antibody specific for the porcine epidemic diarrhea virus spike protein.

ABSTRACT: Porcine epidemic diarrhea (PED) is a diarrheal disease of swine caused by porcine epidemic diarrhea virus (PEDV). It is characterized by acute watery diarrhea, dehydration and vomiting in swine of all ages and is especially fatal for neonatal and postweaning piglets. The spike protein of PEDV plays an important role in mediating virus attachment and fusion to target cells, and recent studies also reported that the neutralizing epitopes of the spike protein were mainly located in the S1 subunit, which makes it a candidate for vaccine development and clinical diagnosis. In this study, we successfully constructed an immune phage display single-domain antibody library with a library size of 3.4 × 10⁶. A single-domain antibody, named S7, specific for the spike protein of PEDV was ide

SUBMITTER: Bao F 

PROVIDER: S-EPMC6626092 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature