Proteomic screen with the proto-oncogene beta-catenin identifies interaction with Golgi coatomer complex I.

ABSTRACT: Beta-catenin is well-known as a key effector of Wnt signalling and aberrant expression is associated with several human cancers. Stabilisation of and atypical subcellular localisation of beta-catenin, regulated in part through specific protein-protein interactions has been linked to cancer development, however the mechanisms behind these pathologies is yet to be fully elucidated. Affinity purification and mass spectrometry were used to identify potential β-catenin interacting proteins in SW480 colon cancer cells. Recombinant β-catenin constructs were used to co-isolate interacting proteins from stable isotope labelled cells followed by detection using mass spectrometry. Several known and new putative interactors were observed. In particular, we identified interaction with a set of coatomer

SUBMITTER: Semaan C 

PROVIDER: S-EPMC6626114 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature