Multi-level immune response network in mild-moderate Chronic Obstructive Pulmonary Disease (COPD).

ABSTRACT:

Background

Chronic Obstructive Pulmonary Disease (COPD) is associated with an abnormal pulmonary and systemic immune response to tobacco smoking. Yet, how do immune cells relate within and between these two biological compartments, how the pulmonary infiltrate influences the lung transcriptome, and what is the role of active smoking vs. presence of disease is unclear.

Methods

To investigate these questions, we simultaneously collected lung tissue and blood from 65 individuals stratified by smoking habit and presence of the disease. The immune cell composition of both tissues was assessed by flow cytometry, whole lung transcriptome was determined with Affymetrix arrays, and we used Weighted Gene Co-expression Network Analysis (WGCNA) to integrate results.

Results

Main

SUBMITTER: Cruz T 

PROVIDER: S-EPMC6626346 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature