Allele-specific real-time PCR testing for minor macrolide-resistant Mycoplasma Pneumoniae.

ABSTRACT:

Background

The point mutations in 23S rRNA gene of Mycoplasma pneumoniae (M. pneumoniae) can lead to high-level resistance to macrolides. This study aimed to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations located at positions A2063G and A2064G of 23S rRNA gene.

Methods

We detected 178 pharyngeal swab specimens and calculated the proportions of resistant and sensitive quasispecies using ASPCR assays. ASPCR assays can detect down to 10 copies of 23S rRNA gene and achieved sensitivities of < 0.1% for A2063G and A2064G. We also compared the findings of ASPCR with the results of nested PCR with sequencing.

Results

Of 178 samples, 164 were found to have M. pneumoniae including 90.85% (149/164) samples with macrolide-resistant M. pn

SUBMITTER: Guo D 

PROVIDER: S-EPMC6626384 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature