Synergistic Effect of Matrix Stiffness and Inflammatory Factors on Osteogenic Differentiation of MSC.

ABSTRACT: Mesenchymal stem cells (MSCs) in vivo reside in a complex microenvironment. Changes of both biochemical and biophysical cues in the microenvironment caused by inflammation affect the differentiation behaviors of MSCs. Most studies, however, only focus on either biochemical or biophysical cues, although the synergistic effect of matrix stiffness and inflammatory factors on osteogenic differentiation of MSCs has not been explored yet. Here, we showed that there was a matrix stiffness-dependent modulation in the osteogenic differentiation of human MSCs (hMSCs) with higher matrix stiffness favoring osteogenesis bias. However, when interleukin-1 β (IL-1β) was added, the osteogenic differentiation of hMSCs was suppressed, which was independent of increasing matrix stiffness. Both experimental ob

SUBMITTER: Wan W 

PROVIDER: S-EPMC6626830 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature